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Male Samuel Knowlton Click to view Samuel Knowlton in the family tree

Samuel was born about 1624 in of Ipswich, Essex, Massachusetts.1   Samuel's father was William Knowlton and his mother was Ann Elizabeth Smith.  His paternal grandparents were Richard Knowlton and Elizabeth Cantize. He had four brothers and a sister, named John, Robert, William, Thomas and Mary.  He was the youngest of the six children.1 

1 phelps.FTW
Further source/citation:
World Family Tree Vol. 1, Ed. 1 @ Tree #0988