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Male James Duke of Rothesay Click to view James Duke of Rothesay in the family tree

James was born on May 22nd, 1540 in St Andrew's,Edinburgh,Mid-Lothian,Scotland.1   James' father was James V Stuart and his mother was Marie Princess Of LORRAINE-GUISE.  His paternal grandparents were James IV Stuart and Sophia VON MECKLENBURG-GÜSTROW ; his maternal grandparents were Claude I De LORRAINE and Antoinette De BOURBON. He had a brother and a sister, named Arthur Prince Of and Lucy.  He was the oldest of the three children. He had nine half-brothers and eleven half-sisters, named John, John, Andrew, Adam, Robert, James III, James I, James, James, Margaret, Anne Or Elizabeth, Christian, Jean, Margaret, Mary, Janet (Jean), Katherine, Elizabeth, Catherine and Jean.  He died in Sterling,Stirlingshire,Scotland,Infant.  He was buried in 1541 in Holyrood Abbey,Edinburgh,Mid-Lothian,Scotland.1 1 

1 Hanover.Frederick.Louis.1707.ancestors.royalfam.ged
Further sources/citations:
Hanover.Frederick.Louis.1707.ancestors.royalfam.ged, GEDCOM File : ~AT92F3.ged