Isabella De Portugal 
Isabella was born on October 4th, 1503 in Lisboa, Lisboa, Portugal.1 She died at the age of 35 on May 1st, 1539 in Toledo, Toledo, Spain.1
Isabella and Charles V were married in a religious ceremony in Palacio Del Alcazar, Sevilla, Sevilla, Spain.1 They had four sons and nine daughters, named Philip Of, Ferdinand, John, Ferdinand, UNKNOWN, Maria, UNKNOWN, Maria X, Mary, UNKNOWN, UNKNOWN, Johanne and Juana.
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Children
Maria Von Hapsburg
Maria was born in 1526 in Spain.1 She died at the age of 50 in 1576 in Frankfurt.1
Philip Of Spain
Philip Of was born on May 21st, 1527 in Valladolid, Valladolid, Spain.1 He died at the age of 71 on September 13th, 1598 in El Escorial, Palace, Madrid, Spain.1
Mary Habsburg
Mary was born on June 21st, 1528 in Madrid, Madrid, Spain.1 She died at the age of 75 in 1603 in Convent Sainte Clara, Villa Monte, Spain.1
Ferdinand DE Castile
Ferdinand was born in 1530 in Madrid, Madrid, Spain.1 He died in 1530 in Madrid, Madrid, Spain.1
Johanne Archduchess
Johanne was born on June 27th, 1537 in Madrid, Madrid, Spain.1 She died at the age of 36 on September 7th, 1573 in Escorial, Madrid, Spain.1
Juana Habsburg
Juana was born on June 27th, 1537 in Madrid, Spain.1 She died at the age of 36 on September 7th, 1573 in Escorial, Madrid, Spain.1
Ferdinand Archduke
Ferdinand was born on April 25th, 1539 in Toledo, Toledo, Spain.1 He died on April 25th, 1539 in Toledo, Toledo, Spain.1
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