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Male James Barlow Click to view James Barlow in the family tree

James was born in 1541 in Prestbury, Cheshire, England.1   James' father was Humphrey Barlow and his mother was Mrs. H. Barlow.  His paternal grandparents were Alexander Barlow and Mrs. A. Barlow. He had two brothers named Henry and William.  He was the second oldest of the three children.1 

Family Details of James' family with Katheryn Barlow

1 phelps.FTW @ Tree #0259
Further source/citation:
World Family Tree Vol. 1, Ed. 1 @ Tree #0259