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Male Samuel Appleton Click to view Samuel Appleton in the family tree

Samuel was born about 1543 in Little, Waldingfield, Suffolk, England.1   Samuel's father was William Appleton and his mother was Rose Sexton.  His paternal grandparents were Robert Appilton and Mary Mounteney; his maternal grandparents were Robert Sexton and Agnes Jermyn. He had two brothers and three sisters, named Thomas, John, Joan, Lacy and Frances.  He was the third oldest of the six children.1 

1 basel.FTW
Further source/citation:
World Family Tree Vol. 9, Ed. 1 @ Tree #2571