Евстафий was born in 1754 in село Тимофеево. Евстафий's father was Сидоров and his mother was Марфа Петровна-Сидорова. His paternal grandfather was Мартын Дмитреевич Сидоров and his paternal grandmother is <Unknown>; his maternal grandparents are <Unknown> and <Unknown>. He has two sisters named Матрёна and Федосья. He is the second oldest of the three children.