James "Younger" Johnstone
James "Younger" was born about 1528 in Johnston, Dunskellie, Dumfries, Scotland.  He died before November 8th, 1567.

 Margaret Hamilton
Margaret was born in Samuelston, East Lothian, Scotland.

 John Johnstone
John was born about 1544 in Dunskellie, Dumfries, Scotland.  He died on June 5th, 1587.