Matthew Stuart
Matthew was born on September 21st, 1516 in Dunbarton Castle, England.  He died at the age of 54 on September 4th, 1571 in Stirling, Stirlingshire, Scotland.

 Margaret Douglas
Margaret was born on October 7th, 1515 in Harbottle Castle, Northumberland.  She died in Hackney, Middlesex, England.  She was buried in Westminster Abbey, London, Middlesex, England.

 Henry Stuart
Henry was born on December 7th, 1545 in Temple Newsham, Yorkshire, England.  He died at the age of 22 in 1567 in Killed at Kirk O'Field.

 Charles Stuart
Charles was born about 1548.