Lord Darnley Henry Stuart
Henry was born in 1545.  He died at the age of 22 in 1567.

 Queen Of Scots Mary Of Scotland
Mary was born in 1542.  She died at the age of 45 in 1587.

 King Of England & Scotland James I Stuart
James I was born in 1566.  He died at the age of 59 in 1625.